Chondrocyte rnaseq
WebCommon methods include extraction of RNA or protein from chondrogenic pellets and measuring levels of chondrocyte markers such as SOX9, aggrecan, type II collagen and others by qPCR or western blotting. ... Isolated RNA may also be used for microarray or RNA-Seq analysis to provide more information on how global gene expression and … WebJun 1, 2024 · scRNA-seq analysis of the condylar segment A reveals that chondrocyte-like cells are scarce but cluster as a distinct population Since we assumed both chondrocyte phenotypes–from AC and GP–to be present in segment A, three samples were separately digested and analysed via scRNA-seq.
Chondrocyte rnaseq
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WebChondrocyte trajectories analysis and IHC staining revealed that transcription factors including TCF4 were found to be highly up-regulated during the occurrence of SIFK, … WebChondrocyte culture is an efficient target system for evaluation of the pharmacological and toxicological effects of drugs acting on cartilaginous tissue. Many types of culture exist …
WebNov 17, 2015 · Tumour necrosis factor up-regulates protein kinase R (PKR)-activating protein (PACT) and increases phosphorylation of PKR and eukaryotic initiation factor 2- in articular chondrocytes. Biochem Soc...
WebThe existing bulk RNA sequencing (bulk RNA-seq) and single-cell sequencing (scRNA-seq) data for chondrocytes in the osteoarthritic knee joint provide the expression profiles of entire cell populations and individual cells, respectively. WebThe existing bulk RNA sequencing (bulk RNA-seq) and single-cell sequencing (scRNA-seq) data for chondrocytes in the osteoarthritic knee joint provide the expression profiles of …
WebSingle-cell RNA-seq of human OA cartilage chondrocytes. (A) Schematic workflow of the experimental strategy. (B) Representative preoperative and postoperative radiographs of …
WebSep 3, 2024 · This article utilizes single-cell RNA-seq data from 22,338 human foreskin keratinocytes to reconstruct the transcriptional regulation of skin development and homeostasis genes, organizing them by differentiation stage and also into transcription factor (TF)–associated modules. hota gold coast parkingWebConclusions: Novel chondrocyte subsets in nucleus pulposus were discovered through scRNA-Seq, which provided novel insight to understand the pathological change during the development of IVDD. Ferroptosis participated in disc degeneration pathogenesis and it might serve as a new target for intervening IVDD. hota hireWebApr 11, 2024 · RNA-Seq Transcriptomic and Quantitative Proteomic Analyses. A total of 9727 transcripts were integrated from the clean data, ... The results collectively suggested a reduced chondrocyte differentiation of the Sr-treated group that supports the positive effect of Sr in the biological function of bone. hota in spanish letterWebAug 23, 2013 · Microarray studies provide insight into cartilage aging but do not reveal the full transcriptomic phenotype of chondrocytes such as small noncoding RNAs, pseudogenes, and microRNAs. RNA-Seq is a powerful technique for the interrogation of large numbers of transcripts including nonprotein coding RNAs. ptb nutcrackerWebSep 20, 2024 · By means of a single cell RNA sequencing on human OA cartilage, they determined the molecular signature of a ferroptotic chondrocyte cluster, which was distinguished by genes involved in oxidative stress, lipid oxidation, Fe2+ response, and ferroptosis, while markers associated with other modalities of cell death were hardly … ptb online shopWebFeb 17, 2024 · Chondrocytes build cartilage in development, governed by the transcription factor SOX9. Using mouse models and transcriptome profiling approaches, we show here that SOX9 also has key roles to maintain growth plates open postnatally and to protect adult articular cartilage from osteoarthritic degradation. hota humanforceWebJan 14, 2024 · This study investigated the early genetic responses of healthy in situ chondrocytes under IL-1β attack with a focus on cell cycle and calcium signaling pathways. RNA sequencing analysis identified... ptb math class 9ex 4.3 solution