Granule-bound starch synthase
WebJun 1, 2008 · Abstract. A rice Wx gene encoding a granule-bound starch synthase I (GBSSI) was introduced into the null-mutant waxy (wx) rice, and its effect on endosperm starches was examined.The apparent amylose content was increased from undetectable amounts for the non-transgenic wx cultivars to 21.6–22.2% of starch weight for the … WebApr 22, 2024 · Using Agrobacterium-mediated transformation, we delivered Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 …
Granule-bound starch synthase
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WebAug 20, 2015 · Jan 2010 - Dec 20156 years. Guelph, Ontario, Canada. My research is on how plant metabolism is regulated, particularly in relation to starch carbohydrate metabolism. My PhD and postdoctoral ... WebDec 28, 2024 · Granule-bound starch synthase (GBSS, encoded by waxy (wx)) is solely responsible for amylose synthesis. Amylopectin biosynthesis requires three coordinated enzymes, including starch-branching enzyme (SBE, encoded by amylose extender1 ( ae1 )), debranching enzyme (DBE, encoded by sugary1 ( su1 )) and starch synthase (SS), …
WebNov 1, 2015 · Total activity of sucrose synthase (SuSy), ADP-glucose pyrophophorylase (AGPase), soluble starch synthase (SS) and granule bound starch synthases (GBSS) during grain filling in wheat; per grain (A) and per mg protein per min (B). Total sucrose and free glucose content (C), and total dry weight and starch (amylose and amylopectin) …
WebApr 11, 2024 · Furthermore, N2 and priming treatments showed higher sink ability to develop grains by showing higher sucrose-to-starch conversion activities of adenosine diphosphate-glucose pyrophosphorylase, uridine diphosphate glucose pyrophosphorylase, sucrose-synthase, soluble-starch synthase, starch branching enzyme and granule … WebJul 12, 2007 · Granule-bound starch synthase I (GBSSI) is one of the key enzymes catalyzing the formation of amylose, a linear α(1,4)D-glucan polymer, from ADP-glucose. Amylose-free transgenic sweet potato plants were produced by inhibiting sweet potato GBSSI gene expression through RNA interference. The gene construct consisting of an …
WebAug 5, 2024 · Gene provides a unified query environment for genes defined by sequence and/or in NCBI's Map Viewer.
WebA rice Wx gene encoding a granule-bound starch synthase I (GBSSI) was introduced into the null-mutant waxy (wx) rice, and its effect on endosperm starches was examined. The … its cjc appWebThe granule-bound starch synthase 1 region showed higher amplification and sequencing success rates, higher interspecific distances, and a perfect barcode gap for the tested species compared to the nuclear internal transcribed spacer 2. Hence, these novel mini-barcodes generated from low copy nuclear gene regions (granule-bound starch … neo outfitWebNov 8, 2024 · Granule-bound starch synthase (GBSS) plays a major role, that of chain elongation, in the biosynthesis of amylose, a starch component with mostly (1 → 4)-α … itscjclark instaWebKey words: granule-bound starch synthase, broomcorn millet, Panicum miliaceum, waxy starch, cereal. Introduction The evolution and diversification of crop plants has been shaped over thousands of years by conscious and uncon-scious human selection on a wide range of phenotypic traits. In plants cultivated primarily as a carbohydrate its circular sawWebThe Wx gene encoding granule-bound starch synthase I (GBSSI) has two major alleles, Wx a and Wx b, which occur predominantly in indica and japonica subspecies, … itsc - it-support center vw.vwgWebFeb 1, 2024 · starch granules were dried with ethanol and acetone after washing, and the amylose content was calculated from the blue value at 680 nm according to the method of Noda et al. (1998). Protein electrophoresis Starch granule-bound proteins were extracted by boiling 60 mg of purified starch granules for 2 min in 460 llof neoorthopraxyWebMay 15, 1999 · Other granule-bound isoforms of starch synthase, such as starch synthase II (SSII), are unable to synthesize amylose. The kinetic properties of GBSSI … neo outside the matrix